The ministry said late Friday that 52 people were injured in the fighting which flared up Wednesday, shattering a UN-brokered cease-fire reached in September that ended hostilities in the city.
The earlier bout of violence killed nearly 100 people.
In a statement, the ministry appealed to rival militiamen not to target ambulances and medics.
The fighting between militias allied to the UN-backed government in Tripoli and an armed group from a nearby town underscores Libya’s lingering lawlessness since the 2011 uprising that toppled and killed Moammar Gadhafi.
The energy-rich North African nation is governed by rival authorities in Tripoli and the country’s east, each of which is backed by an array of militias.